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Professor R. Andres Floto MA PhD FRCP

Contact Details

Phone 01480 364673 (sec)
Fax 01480 364330

Specialist Register

Respiratory Medicine (adults)

Specialist interests

Cystic Fibrosis (CF)
Non-CF Bronchiectasis
Lung infection (TB, Non Tuberculous Mycobacteria (NTM) and Aspergillus)
Primary and Secondary Immunodeficiencies

Research interests

Dendritic cell and macrophage biology
Mycobacterial-immune cell interactions
Pneumococcal infection and immunity
Metabolic control of lung inflammation
Innate Immunity to bacterial infections See Floto Lab for more details

Professional positions

Professor of Respiratory Biology, University of Cambridge
Principal Investigator, Cambridge Institute for Medical Research, University of Cambridge
Research Director, Cambridge Centre for Lung Infection
Honorary Consultant, Papworth Hospital
Honorary Consultant, Addenbrooke’s Hospital
Co-chair of the British Thoracic Society NTM Guidelines Committee
Co-chair US CF Foundation-European CF Society NTM Guidelines Group
Co-chair ECFS Working Group on NTM

Professional Membership

Member, European Respiratory Society
Member, American Thoracic Society
Member, Association of Physicians of Great Britain and Ireland
Fellow, Royal College of Physicians UK
Member, European CF Society

Education and Training

1991 BA University of Cambridge
1995 MA University of Cambridge
1997 PhD University of Cambridge
1997 MB BChir University of Cambridge
2000 MRCP London
2007 Completion of specialist respiratory training
2013 FRCP London

Selected Publications

Cader MZ, Boroviak K, Zhang Q, Assadi G, Kempster SL, Sewell GW, Saveljeva S, Ashcroft JW, Clare S, Mukhopadhyay S, Brown KP, Tschurtschenthaler M, Raine T, Doe B, Chilvers ER, Griffin JL, Kaneider NC, Floto RA, D'Amato M, Bradley A, Wakelam MJ, Dougan G, Kaser A. (2016). C13orf31 (FAMIN) is a central regulator of immunometabolic function. Nat Immunol. 17: 1046-56.

Viviani L, Harrison MJ, Zolin A, Haworth CS, Floto RA. (2016). Epidemiology of nontuberculous mycobacteria (NTM) amongst individuals with cystic fibrosis (CF). J. Cyst Fibros. pii: S1569-1993(16)30008-X.
Klionsky DJ….Floto RA et al. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 12: 1-222.

Floto RA, Olivier KN, Saiman L, Daley CL, Herrmann J-L, Nick JA, Noone PG, Bilton D, Corris P, Gibson RL, Hempstead SE, Koetz K, Sabadosa KA, Sermet-Gaudelus I, Smyth AR, van Ingen J, Wallace RJ, Winthrop KL, Marshall BC, Haworth CS (2015). Cystic Fibrosis Foundation and European Cystic Fibrosis Society Consensus Recommendations for the Management of Nontuberculous Mycobacteria in Individuals with Cystic Fibrosis. Executive Summary. Thorax 2016; 71:88-90.

Floto RA, Olivier KN, Saiman L, Daley CL, Herrmann J-L, Nick JA, Noone PG, Bilton D, Corris P, Gibson RL, Hempstead SE, Koetz K, Sabadosa KA, Sermet-Gaudelus I, Smyth AR, van Ingen J, Wallace RJ, Winthrop KL, Marshall BC, Haworth CS (2015). Cystic Fibrosis Foundation and European Cystic Fibrosis Society Consensus Recommendations for the Management of Nontuberculous Mycobacteria in Individuals with Cystic Fibrosis. Thorax 2016; 71 Suppl 1: i1-i22

Crousilles A, Maunders E, Bartlett S, Fan C, Ukor EF, Abdelhamid Y, Baker Y, Floto A, Spring DR, Welch M. (2015) Which microbial factors really are important in Pseudomonas aeruginosa infections? Future Microbiol. 10, 1825-36.

Panagea T, Pincus DH, Grogono D, Jones M, Bryant J, Parkhill J, Floto RA, Gilligan P (2015). Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. J Clin Microbiol. 53, 2355-8.

Parkins MD, Floto RA. (2015) Emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis. J Cyst Fibros. 14, 293-304.

Lourenco S, Teixeira VH, Kalber T, Jose RJ, Floto RA, Janes SM. (2015). Macrophage migration inhibitory factor-CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors. J Immunol. 194, 3463-74.

Floto RA, Haworth CS. The growing threat of nontuberculous mycobacteria in CF. (2015) J Cyst Fibros. 14, 1-2.

*Hepburn L, Prajsnar TK, Klapholz C, Moreno P, Loynes CA, Ogryzko NV, Brown K, Schiebler M, Hegyi K, Antrobus R, Hammond KL, Connolly J, Ochoa B, Bryant C, Otto M, Surewaard B, Seneviratne SJ, Grogono DM, Cachat J, Ny T, Kaser A, Török ME, Peacock SJ, Holden M, Blundell T, Wang L, Ligoxygakis P, Minichiello L, Woods CG, Foster SJ, Renshaw SA, Floto RA (2014). A Spaetzle-like role for Nerve Growth Factor  in vertebrate immunity to Staphylococcus aureus. Science 346, 641-646.

Schiebler M, Brown K, Hegyi K, Newton SM, Renna M, Hepburn L, Schaffner C, Coulter S, Obregón A, Henao Tamayo M, Basaraba R, Ordway DJ Kampmann B, Anderson KE, Hawkins PT, Rubinsztein DC, Floto RA (2014). Anticonvulsants stimulate mTOR-independent autophagic killing of Mycobacterium tuberculosis through inositol depletion.  EMBO Molecular Medicine 7, 127-139.

Attout T, Floto A, Launay P (2014) Calcium channels in Fc receptor signaling. Curr Top Microbiol Immunol. 382:95-110.

Segovia M, Louvet C, Charnet P, Savina A, Tilly G, Gautreau L, Carretero-Iglesia L, Beriou G, Cebrian I, Cens T, Hepburn L, Chiffoleau E, Floto RA, Anegon I, Amigorena S, Hill M, Cuturi MC. (2014). Autologous dendritic cells prolong allograft survival through Tmem176b-dependent antigen cross-presentation. Am J Transplant. 14:1021-31.

Hiemstra TF, Charles PD, Gracia T, Hester SS, Gatto L, Al-Lamki R, Floto RA, Su Y, Skepper JN, Lilley KS, Karet Frankl FE. (2014) Human urinary exosomes as innate immune effectors. J Am Soc Nephrol. 25:2017-27.

*Bryant JM, Grogono DM, Greaves D, Foweraker J, Roddick I, Inns T, Reacher M, Haworth CS, Curran MD, Harris SR, Peacock SJ, Parkhill J, Floto RA (2013). Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study. Lancet.  381: 1551-60. PMC3664974

Mueller M, Barros P, Witherden AS, Roberts AL, Zhang Z, Schaschl H, Yu CY, Hurles ME, Schaffner C, Floto RA, Game L, Steinberg KM, Wilson RK, Graves TA, Eichler EE, Cook HT, Vyse TJ, Aitman TJ (2013) Genomic pathology of SLE-associated copy-number variation at the FCGR2C/FCGR3B/FCGR2B locus. Am J Hum Genet 92:28-40.

Stout JE, Floto RA (2012) Treatment of Mycobacterium abscessus: all macrolides are equal but perhaps some are more equal than others. Am J Respir Crit Care Med 186: 822-3.

Hill UG, Floto RA, Haworth CS (2012).  Non-tuberculous mycobacteria in cystic fibrosis. J R Soc Med 105,, S14-18.

Mason MJ, Schaffner C, Floto RA, Teo QA. (2011) Constitutive Expression of a Mg2+ Inhibited K+ Current and a TRPM7-like Current in Human Erythroleukemia Cells. Am J Physiol Cell Physiol. (in press) PMID: 22135214

Renna M, Schaffner C, Brown K, Shang S, Tamayo MH, Hegyi K, Grimsey NJ, Cusens D, Coulter S, Cooper J, Bowden AR, Newton SM, Kampmann B, Helm J, Jones A, Haworth CS, Basaraba RJ, DeGroote MA, Ordway DJ, Rubinsztein DC, Floto RA. (2011). Azithromycin blocks autophagy and may predispose cystic fibrosis patients to mycobacterial infection. J Clin Invest. 121, 3554-63.

Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC. (2011) Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex. J Cell Sci. 124:469-82.

Dai X, Jayapal M, Tay HK, Reghunathan R, Lin G, Too CT, Lim YT, Chan SH, Kemeny DM, Floto RA, Smith KG, Melendez AJ, MacAry PA. (2009). Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa. Blood 114, 318-27.

Sarkar S, Ravikumar B, Floto RA, Rubinsztein DC. (2008). Rapamycin and mTOR-independent autophagy inducers ameliorate toxicity of polyglutamine-expanded huntingtin and related proteinopathies. Cell Death Differ 16, 46-56.

Williams A, Sarkar S, Cuddon P, Ttofi EK, Saiki S, Siddiqi FH, Jahreiss L, Fleming A, Pask D, Goldsmith P, O'Kane CJ, Floto RA & Rubinsztein DC. (2008). Novel targets for Huntington's disease in an mTOR-independent autophagy pathway. Nat Chem Biol. 4, 295-305.

Brownlie RJ, Lawlor KE, Niederer HA, Cutler AJ, Xiang Z, Clatworthy MR, Floto RA, Greaves DR, Lyons PA. & Smith KG.(2008).Distinct cell-specific control of autoimmunity and infection by FcgammaRIIb. J Exp Med. 205, 883-895.

Floto, R.A., Sarkar, S, Perlstein, E.O., Kampmann, B., Schreiber, S.L. & Rubinsztein, D.C. (2007). Small molecule enhancers of rapamycin-induced TOR inhibition promote autophagy, reduce toxicity in Huntington’s disease models and enhance killing of mycobacteria by macrophages. Autophagy 3, 620-622.

Martineau, A.R., Wilkinson, K.A., Newton, S.M., Floto, R.A., Norman, A.W., Skolimowska, K., Davidson, R.N., Sorensen, O.E., Kampmann, B., Griffiths, C.J. & Wilkinson, R.J. (2007). IFN-gamma and TNF-inderpendent vitamin D-inducible human suppression of mycobacteria: the role of cathelicidin LL-37. J. Immunol. 178, 7190-7198.

Newland, S.A., Macaulay, I.C., Floto, R.A., de Vet, E. C., Ouwehand, W.H., Watkins, N.A., Lyons, P.A. & Campbell, R.D. (2007). The novel inhibitory receptor G6B is expressed on the surface of platelets and attenuates platelet function in vitro. Blood. 109, 4806-4809.

Clatworthy, M.R., Willcocks, L., Urban, B.,Langhorne, J., Williams, T., Watkins, N.A., Rankin, A., Floto, R.A. & Smith, K.G.C. (2007). SLE-associated defects in the inhibitory receptor Fc RIIb reduce susceptibility to malaria. Proc Natl Acad Sci U S A. 104, 7169-7174.

*Floto, R.A., MacAry, P.A., Boname, J.M., Mien, T.S., Houben, E.N.G., Pieters, J., Day, C., Oehlmann, W., Singh, M., Smith, K.G.C. & Lehner, P.J. (2006). Dendritic cell stimulation by mycobacterial HSP70 is mediated through the HIV co-receptor CCR5. Science 314, 454-458.

Floto, R.A., Clatworthy, M.R., Heilbronn, K.R., Rosner, D.R., MacAry, P.A., Rankin, A., Lehner, P.J., Ouwehand, W.H., Allen, J.M., Watkins, N.A. & Smith, K.G.C (2005). An SLE-associated polymorphism of the inhibitory receptor Fc RIIb excludes it from lipid rafts and leads to loss of function. Nature Medicine, 11, 1056-1058. Corresponding author.

Sarkar, S., Floto, R.A., Berger, Z., Imarisio, S., Cordenier, A., Pasco, M., Cook, L.J. & Rubinsztein, D.C. (2005). Lithium induces autophagy by inhibiting inositol monophosphatase. J. Cell Biol. 170, 1101-1111

Barrow, A.D., Astoul, E., Floto, R.A., Brooke, G., Ingrid A., Relou, I.A., Foad, N., Smith, K.G.C., Barclay. N., Ouwehand, W., Farndale, R.W., Alexander, D.R. & Trowsdale, J. (2004).  Human TREM-like transcript-1 is a platelet specific ITIM containing co-activating receptor. J. Immunol. 172, 5838-5842.

MacAry, P.A., Javid, B., Floto, R.A., Smith, K.G.C., Singh, M. & Lehner, P.J. (2004). HSP70 Peptide-binding mutants separate antigen delivery from dendritic cell stimulation. Immunity 20, 95-106

Floto, R.A. & Smith, K.G.C. (2003).  The vagus nerve, macrophages and nicotine. Lancet, 361, 1069-1070. Corresponding author.

Melendez, A.R., Bruetschy, L., Floto, R.A., Harnett, M.M. & Allen, J.M. (2001). Functional coupling of Fc RI to NADPH oxidative burst and immune complex trafficking requires the activation of phospholipase D1. Blood 98, 3421-3428.

Melendez, A.R., Floto, R.A., Gillooly, D.J., Harnett, M.M.& Allen, J.M. (1998). Fc RI-coupling to phospholipase D initiates sphingosine kinase-mediated calcium mobilisation and vesicular trafficking. J.Biol.Chem. 273, 9393-9402.

Melendez, A.R., Floto, R.A., Cameron, A.J., Gillooly, D.J., Harnett, M.M. & Allen, J.M. (1998). Molecular switch by Fc RI antibody receptor mobilises calcium through two distinct signalling pathways. Current Biology 8, 210-221.

Norman, J.C., Harrison, P.T., Davis, W., Floto, R.A. & Allen, J.M. (1998). Vesicular trafficking of FcRI from an early endosome compartment requires recruitment of tyrosine kinases. Immunology 94, 48-55.

Floto, R.A., Somasundaram, B., Allen, J.M. & Mahaut-Smith, M.P. (1997) FcRI activation triggers a novel Ca2+-activated monovalent cation channel in the human monocytic cell line, U937. J.Biol.Chem. 272, 4753-4758. Corresponding author.

Somasundaram, B., Mahaut-Smith, M.P. & Floto, R.A.(1996).Temperature-dependent block of capacitative Ca2+ influx in the human leukaemic cell line KU-812. J.Biol.Chem. 271, 26096-26104.

Floto, R.A., Mahaut-Smith, M.P., Allen, J.M. & Somasundaram, B.(1996).Differentiation of the human monocytic cell line, U937, results in the upregulation of the calcium release activated current, ICRAC J.Physiol. 495, 331-338. Corresponding author.

Floto, R.A., Somasundaram, B., Mahaut-Smith, M.P. & Allen, J.M.  (1995). IgG-Induced Ca2+ oscillations in differentiated U937 cells: a study using laser scanning confocal microscopy and co-loaded Fluo3 and Fura-Red. Cell Calcium 18, 377-389. Corresponding author.

Hutchinson, M.J., Harrison, P.T., Floto, R.A. & Allen, J.M. (1995) Fc receptor-mediated phagocytosis requires tyrosine kinase activity and is ligand independent Eur.J.Immunol. 25, 481-487

Book Chapters:
Floto R.A. & Haworth CS. (2011) Bronchiectasis European Respiratory Society Monograph  (Editor)

Floto, R.A. (2010) Chapter (‘Cell Signalling’), Oxford Textbook of Medicine.5th Edition.

Floto, R.A. (2008) Chapter (‘Respiratory Physiology and Medicine’) Pastest, UK.

Floto, A, Overington, J. & Allen, J. (2004). Pleiotropic effects of statins. Annual Reports in Medicinal Chemistry 39, 241-258.